Scientist, Platform Development
Department: 103 PlatformLocation: Waltham, MA
Company Overview:
Dyne Therapeutics is building a leading muscle disease company dedicated to advancing innovative life-transforming therapeutics for people living with genetically driven diseases. With its proprietary FORCE™ platform, Dyne is developing modern oligonucleotide therapeutics that are designed to overcome limitations in delivery to muscle tissue seen with other approaches. Dyne’s broad portfolio of therapeutic candidates for serious muscle diseases includes programs for myotonic dystrophy type 1 (DM1), Duchenne muscular dystrophy (DMD) and facioscapulohumeral muscular dystrophy (FSHD). For more information, please visit https://www.dyne-tx.com/, and follow us on Twitter, LinkedIn and Facebook.
Role Summary:
Dyne is seeking a Scientist – Platform Development. The Scientist – Platform Development will collaborate closely with in vitro/in vivo biology colleagues to generate and characterize research materials and will also help establish and contribute to the operations of our laboratories.
This role is based in Waltham, Massachusetts, without the possibility of being a remote role.
Primary Responsibilities Include:
- Design, optimize and execute protein conjugations on multiple proteins and multiple payloads- including NHS ester, maleimide, ranging from milligram to gram scale.
- Using AKTA systems, perform purifications on protein conjugates on multiple proteins and multiple payloads, ranging from milligram to gram scale.
- Activate multiple payloads with various linkers from milligram to gram scale.
- Purify multiple active linker-payloads from milligram to gram scale.
- Perform biochemical analysis on in-process and final purified conjugates, including the following techniques: Mass Spectrometry (MS), CE-IEF, CE-SDS, SDS-PAGE, BCA, SEC-HPLC, and uv-vis.
- Perform Tangential Flow Filtration (TFF; UF/DF) from the milliliter to multi-Liter scale.
- Serve as project lead and subject matter expert (SME) in both in-house and external meetings and communications.
- Serve as a mentor to junior lab members and/or co-ops.
- Contribute to company intellectual property (IP) by communicating, in both spoken and written terms, technical details to IP attorneys.
- Write technical reports to support technical transfer(s) to CDMOs.
- Contribute to critical discussions of therapeutic structure, function, and new targets across all company levels.
- Maintain an electronic lab notebook in a timely manner.
Education & Skill Requirements
BS or equivalent in Chemistry or Biochemistry and 2 years of experience in drug discovery and development OR MS in Chemistry or Biochemistry with coursework or research in drug discovery and development.
Other Requirements:
- Must have contributed to at least 1 scientific publication
- Must have demonstrated proficiency in each of the following:
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- Conjugation techniques- including NHS Ester and Maleimide chemistry
- Purifying, analyzing and characterizing conjugates
- Using preparative purification systems
- Chromatographic separation (e.g. SEC, HIC, IEX)
- Mass Spectrometry (MS)
- UPLC/HPLC
- Protein biochemical assays- including SDS-PAGE, ELISA, and uv-vis
#LI-Onsite
The statements contained herein reflect general details as necessary to describe the principles functions for this job, the level of knowledge and skill typically required, and the scope of responsibility, but should not be considered an all-inclusive listing of work requirements. Individuals may perform other duties as assigned, including work in other functional areas to cover absences or relief, to equalize peak work periods or otherwise balance workload.
This description is not intended to be constructed as an exhaustive list of duties, responsibilities, or requirements for the position. This position may change or assume additional duties at any time. The employee may be requested to perform different or additional duties as assigned. All Employees are expected to adhere to all company policies and act as a role model for company values.
Dyne Therapeutics is an equal opportunity employer and will not discriminate against any employee or applicant on the basis of age, color, disability, gender, national origin, race, religion, sexual orientation, veteran status, or any classification protected by federal, state, or local law.
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